Testing honey antibacterial activity by determining the minimal inhibitory concentration (MIC)
Determination of the minimal inhibitory concentration (MIC) of honey provides an important measurement for the overall antibacterial efficacy of honey samples. In other words, the MIC represents the lowest possible concentration of the honey sample at which bacterial growth is suppressed / inhibited. The lower MIC value (number), the higher the content of antibacterial compounds and the greater overall antibacterial activity.
Lower MIC number of honey = the greater antibacterial activity of the tested honey
There will be three MIC values expressing the antibacterial potential of honey: 4.5, 9.0 and 18.0. The values reflect the percentage of honey sample solution sufficient to inhibit the bacterial growth of a model bacterial strain - Staphylococcus aureus. The average MIC value of artificial honey (sugars solution only) against model bacterium is 42.5. If the MIC number of honey sample is closer to the MIC of artificial honey, the honey sample is less effective. This may indicate detrimental changes to honey antibacterial potential due to prolonged storage or uncontrolled heating.
How to test your honey
For the stability of antibacterial activity, the Honey laboratory applies the following conditions before testing samples:
- Only the sample of honey in a jar (any volume) in its final commercial form will be subjected for testing.
- The honey sample delivered in person or by post directly to the contact address of the Honey Laboratory (Institute of Molecular Biology SAS, Dúbravska cesta 21, 84551 Bratislava, Slovakia).
- Along with the sample of honey, it is necessary to send a declaration of oath (template available to download below) on the number of filled jars from one tested batch.
- Quality marks (GOLD, SILVER, BRONZE) accompanied with study protocol will be awarded to processed honey samples that meet our internal qualitative requirements based on MIC values. Tested honey samples which do not meet our requirements for quality marks will be issued a protocol with results from analysis without quality mark.
- Quality marks will be charged additionally to the price of analysis and will be directly provided by Honey laboratory.
- Honey samples provided for analysis will not be returned, but will be stored and kept for possible retrospective control analysis of issued quality marks or alternatively for scientific purposes.
- The Honey laboratory reserves the right to refuse the performance of sample analysis due to non-compliance with the above conditions or in case of misuse of the quality mark.
Test MIC - 1 sample
Analysis of 1 honey sample
Test MIC - 5 samples
Analysis of 5 honey samples (discount price)